Characterisation of a Bacterium from Selat Tebrau and Screening of Microbial Genomes for Dehalogenases

Current study was to investigate the presence of dehalogenase in isolated bacterium from Selat Tebrau that can grow on 2,2-dichloropropionic acid (2,2-DCP is an active compound herbicide Dalapon®). Strain RN1, a Gram-negative and rod shape tentatively identified as Enterobacter cancerogenus based basic biochemical 16S rRNA gene analyses. The calculated cells doubling time were 5.29 hours growth liquid minimal media with 10, 20 30 mM 2,2-DCP, respectively. However, no observed at 40 2,2-DCP medium due increase toxicity. It hypothesized that, strain RN1 produced dehalogenase(s) which merits further genomic data same genus species available database. A putative dehalogenase, designated DehRN located published cancerogenus. Pairwise amino acids known resulted sequence identity <20%, suggesting new class enzyme cancerogenus.Keywords: dichloropropionate, Enterobacter, haloalkanoic